6UAK

LahSb - C-terminal methyltransferase involved in RiPP biosynthesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-03
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.12723
Spacegroup name	P 32 2 1
Unit cell lengths	115.729, 115.729, 71.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.010
R-factor	0.2404
Rwork	0.239
R-free	0.27680
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.583
Data reduction software	XDS
Data scaling software	pointless
Phasing software	SHARP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.200	2.040
High resolution limit [Å]	2.010	2.010
Number of reflections	34750	1825
<I/σ(I)>	20.8
Completeness [%]	94.1
Redundancy	20
CC(1/2)	0.999	0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		282	0.2 M sodium malonate, 16-22% of PEG3350