6UAG
Closed Dimer of Y77A Mutant Putative Ryanodine Receptor from Bacteroides thetaiotaomicron VPI-5482
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.968, 89.391, 74.927 |
| Unit cell angles | 90.00, 111.17, 90.00 |
Refinement procedure
| Resolution | 37.657 - 2.709 |
| R-factor | 0.1993 |
| Rwork | 0.196 |
| R-free | 0.26360 |
| Structure solution method | SAD |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
| Rmerge | 0.080 | 0.033 | 0.629 |
| Total number of observations | 77014 | ||
| Number of reflections | 24972 | 1245 | 1223 |
| <I/σ(I)> | 8.7 | ||
| Completeness [%] | 99.0 | 95.2 | 99.6 |
| Redundancy | 3.1 | 2.9 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 1.5M sodium citrate |
