6UAG

Closed Dimer of Y77A Mutant Putative Ryanodine Receptor from Bacteroides thetaiotaomicron VPI-5482

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97927
Spacegroup name	P 1 21 1
Unit cell lengths	74.968, 89.391, 74.927
Unit cell angles	90.00, 111.17, 90.00

Refinement procedure

Resolution	37.657 - 2.709
R-factor	0.1993
Rwork	0.196
R-free	0.26360
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.080	0.033	0.629
Total number of observations	77014
Number of reflections	24972	1245	1223
<I/σ(I)>	8.7
Completeness [%]	99.0	95.2	99.6
Redundancy	3.1	2.9	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	297	1.5M sodium citrate