6UAF
Crystal Structure of the Metallo-beta-lactamase L1 from Stenotrophomonas maltophilia in the Complex with Hydrolyzed Imipnem
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-02-21 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 104.835, 104.835, 98.627 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.310 - 1.900 |
| R-factor | 0.1738 |
| Rwork | 0.172 |
| R-free | 0.20760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 6U0Y |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.780 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.109 | 0.692 |
| Number of reflections | 26020 | 1258 |
| <I/σ(I)> | 40.9 | 6.29 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.2 | 20.4 |
| CC(1/2) | 0.997 | 0.966 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2 M sodium malonate pH 7.0, 20 % w/v PEG3350 |
