6UAC
Crystal Structure of the metallo-beta-lactamase L1 from Stenotrophomonas maltophilia in the complex with cadmium and hydrolyzed moxolactam
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-21 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 104.339, 104.339, 98.975 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.400 - 1.600 |
| R-factor | 0.1549 |
| Rwork | 0.154 |
| R-free | 0.17300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 6U0Y |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.832 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.138 | 0.901 |
| Number of reflections | 42294 | 1952 |
| <I/σ(I)> | 31.3 | 1.45 |
| Completeness [%] | 99.6 | 94 |
| Redundancy | 19.1 | 9.1 |
| CC(1/2) | 0.593 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2 M sodium malonate pH 7.0, 20 % w/v PEG3350 |
