6UA1
Crystal Structure of the metallo-beta-lactamase L1 from Stenotrophomonas maltophilia in the no-metal bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-22 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 104.467, 104.467, 99.035 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.150 - 1.800 |
| R-factor | 0.1927 |
| Rwork | 0.191 |
| R-free | 0.22430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 6U0Y |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.775 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.119 | 0.898 |
| Number of reflections | 29676 | 1215 |
| <I/σ(I)> | 31 | 0.67 |
| Completeness [%] | 98.7 | 83.2 |
| Redundancy | 14.6 | 6.7 |
| CC(1/2) | 0.552 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2 M sodium malonate pH 7.0, 20 % w/v PEG3350 |
