6U9Z
Wild-type MthK pore in 6 mM K+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.009 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 63.811, 63.811, 44.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.050 - 1.954 |
| R-factor | 0.2067 |
| Rwork | 0.205 |
| R-free | 0.22630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ldc |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.433 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.050 | 2.024 |
| High resolution limit [Å] | 1.954 | 1.954 |
| Rmerge | 0.186 | 2.100 |
| Rmeas | 0.190 | 2.148 |
| Rpim | 0.038 | 0.448 |
| Number of reflections | 6986 | 660 |
| <I/σ(I)> | 15.95 | 1.57 |
| Completeness [%] | 99.7 | 97.05 |
| Redundancy | 24.7 | 22.5 |
| CC(1/2) | 0.999 | 0.751 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | protein (in 100 mM sodium chloride, 1 mM potassium chloride, 10 mM MOPS) + 3.5-4.0 M 1,6-hexanediol, 100 mM MES-NaOH, pH 6.75, 5 mM potassium chloride |
