6U9X
Structure of T. brucei MERS1-RNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.445, 89.360, 71.351 |
| Unit cell angles | 90.00, 109.51, 90.00 |
Refinement procedure
| Resolution | 74.391 - 2.600 |
| R-factor | 0.2207 |
| Rwork | 0.218 |
| R-free | 0.24990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6p5r |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.684 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.391 | 2.665 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rpim | 0.074 | 0.389 |
| Number of reflections | 23984 | 1259 |
| <I/σ(I)> | 5.7 | 1.4 |
| Completeness [%] | 92.3 | 72.3 |
| Redundancy | 2.5 | |
| CC(1/2) | 0.981 | 0.839 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M N-cyclohexyl-3-aminopropanesulfonic acid (CAPS) pH 10.5, 800 mM Potassium phosphate dibasic, 1.2 M sodium phosphate monobasic, 0.2 M lithium sulphate |
