6U9U

Structure of GM9_TH8seq732127 FAB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-08-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	147.709, 147.709, 63.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.783 - 2.260
R-factor	0.2102
Rwork	0.208
R-free	0.24950
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.260	2.260
Number of reflections	33355	1625
<I/σ(I)>	27.8
Completeness [%]	100.0
Redundancy	17.9
CC(1/2)	0.999	0.837

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	4M Sodium Formate