6U8Z
Crystal Structure of Catalytic Domain of Human Phospholipase D1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X17B1 |
| Synchrotron site | NSLS |
| Beamline | X17B1 |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2017-11-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 75.209, 75.209, 239.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.759 - 1.799 |
| R-factor | 0.1751 |
| Rwork | 0.174 |
| R-free | 0.21450 |
| Structure solution method | SAD |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 79.880 | 79.960 | 1.830 |
| High resolution limit [Å] | 1.799 | 4.880 | 1.800 |
| Rmeas | 0.158 | 0.096 | 2.533 |
| Rpim | 0.053 | 0.033 | 0.850 |
| Total number of observations | 28866 | 27207 | |
| Number of reflections | 64302 | 3611 | 3147 |
| <I/σ(I)> | 7 | 18 | 0.8 |
| Completeness [%] | 99.0 | 100 | 98.1 |
| Redundancy | 8.7 | 8 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 298 | 0.2 M ammonium citrate, 15% PEG3350, pH 5.3 |
