6U8X

Crystal structure of DNMT3B-DNMT3L in complex with CpApG DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9774
Spacegroup name	P 31
Unit cell lengths	193.845, 193.845, 49.884
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.883 - 2.951
R-factor	0.228294539359
Rwork	0.227
R-free	0.25151
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yx2
RMSD bond length	0.003
RMSD bond angle	0.621
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.883	3.060
High resolution limit [Å]	2.950	2.950
Number of reflections	43984	4359
<I/σ(I)>	8.8
Completeness [%]	99.6
Redundancy	5.2
CC(1/2)	0.998	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	289.15	0.1 M Tris-HCl (pH 8.0), 100 mM MgCl2, 7% PEG8000