6U8F

RNA hairpin, bound with TNA monomer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	99
Detector technology	CCD
Collection date	2017-06-09
Detector	MAR CCD 130 mm
Wavelength(s)	1
Spacegroup name	H 3
Unit cell lengths	69.631, 69.631, 70.514
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.830 - 2.810
R-factor	0.176
Rwork	0.175
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vci
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.100	0.567
Rmeas	0.111	0.647
Rpim	0.047	0.304
Number of reflections	3110	315
<I/σ(I)>	15.7	1.42
Completeness [%]	99.5	96
Redundancy	5.4	4.1
CC(1/2)	0.988	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	1 M Lithium sulfate, 50 mM tri-Sodium citrate, 3 % w/v 2-Propanol, 50 mM HEPES; pH 7.5