6U7Z

RNA hairpin structure containing one TNA nucleotide as primer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	99
Detector technology	CCD
Collection date	2019-05-28
Detector	MAR CCD 130 mm
Wavelength(s)	1
Spacegroup name	H 3
Unit cell lengths	71.319, 71.319, 67.566
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.590 - 2.710
R-factor	0.173
Rwork	0.170
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vci
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.117	0.415
Rmeas	0.137	0.500
Rpim	0.071	0.274
Number of reflections	3369	305
<I/σ(I)>	9.48	2.3
Completeness [%]	97.5	89.4
Redundancy	3.5	2.8
CC(1/2)	0.986	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	3.5 M Sodium formate pH 7.0