6U7Y

RNA hairpin structure containing one TNA nucleotide as template

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	99
Detector technology	CCD
Collection date	2019-08-15
Detector	MAR CCD 130 mm
Wavelength(s)	1
Spacegroup name	H 3
Unit cell lengths	68.766, 68.766, 69.960
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.350 - 2.950
R-factor	0.186
Rwork	0.182
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vci
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.060
High resolution limit [Å]	2.950	2.950
Rmerge	0.116	0.332
Rmeas	0.141	0.413
Rpim	0.079	0.243
Number of reflections	2599	263
<I/σ(I)>	10.3	1.83
Completeness [%]	98.5	89.6
Redundancy	6	4.5
CC(1/2)	1.000	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.2 M Lithium sulfate monohydrate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350