6U7Y
RNA hairpin structure containing one TNA nucleotide as template
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2019-08-15 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 68.766, 68.766, 69.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.350 - 2.950 |
| R-factor | 0.186 |
| Rwork | 0.182 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vci |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.060 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.116 | 0.332 |
| Rmeas | 0.141 | 0.413 |
| Rpim | 0.079 | 0.243 |
| Number of reflections | 2599 | 263 |
| <I/σ(I)> | 10.3 | 1.83 |
| Completeness [%] | 98.5 | 89.6 |
| Redundancy | 6 | 4.5 |
| CC(1/2) | 1.000 | 0.956 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2 M Lithium sulfate monohydrate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350 |
