6U7L
2.75 Angstrom Crystal Structure of Galactarate Dehydratase from Escherichia coli.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.462, 167.574, 117.065 |
| Unit cell angles | 90.00, 103.97, 90.00 |
Refinement procedure
| Resolution | 29.230 - 2.750 |
| R-factor | 0.2082 |
| Rwork | 0.206 |
| R-free | 0.24090 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.198 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.100 | 0.793 |
| Rmeas | 0.109 | 0.867 |
| Rpim | 0.044 | 0.349 |
| Number of reflections | 56199 | 2851 |
| <I/σ(I)> | 16.7 | 2.2 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 6.1 | 6.2 |
| CC(1/2) | 0.779 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 292 | Protein: 7.6 mg/ml, 0.5 Sodium chloride, 0.01M Tris pH 8.3; Screen: PACT (D11), 0.2M Calcium chloride, 0.1M Tris pH 8.0, 20% (w/v) PEG 6000. |
