6U7I

Faecalibacterium prausnitzii Beta-glucuronidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	122.597, 129.055, 161.424
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.330 - 2.700
R-factor	0.1828
Rwork	0.180
R-free	0.27340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5z1b
RMSD bond length	0.009
RMSD bond angle	1.099
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.330	2.799
High resolution limit [Å]	2.700	2.702
Number of reflections	66748	6563
<I/σ(I)>	7.71
Completeness [%]	91.8
Redundancy	4.6
CC(1/2)	0.969	0.708

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Bis-Tris: HCl, pH 8.5 and 1.8 M Magnesium Sulfate