6U7F

HCoV-229E RBD Class IV in complex with human APN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-01-21
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	99.158, 98.572, 147.778
Unit cell angles	90.00, 104.42, 90.00

Refinement procedure

Resolution	49.382 - 2.750
R-factor	0.1922
Rwork	0.190
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fyq
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16rc1_3535)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.382	49.382	2.820
High resolution limit [Å]	2.750	12.280	2.750
Rmerge	0.068	0.024	0.448
Rmeas	0.079	0.028	0.521
Total number of observations	273221
Number of reflections	72016	840	5288
<I/σ(I)>	16.24	38.97	3.51
Completeness [%]	99.8	97.9	100
Redundancy	3.794	3.562	3.846
CC(1/2)	0.998	0.998	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	9% PEG8000, 1 mM oxidized glutathione, 1 mM reduced glutathione, 5% glycerol, 100 mM MES, pH 6.5