6U6O
Crystal structure of a vaccine-elicited anti-HIV-1 rhesus macaque antibody DH846
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-08-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.018, 36.991, 87.921 |
| Unit cell angles | 90.00, 111.96, 90.00 |
Refinement procedure
| Resolution | 43.844 - 2.806 |
| R-factor | 0.2152 |
| Rwork | 0.212 |
| R-free | 0.27230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6u6m |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.693 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmeas | 0.757 | |
| Rpim | 0.437 | |
| Number of reflections | 9288 | 1097 |
| <I/σ(I)> | 5.2 | 1.6 |
| Completeness [%] | 85.2 | |
| Redundancy | 2.7 | 2.6 |
| CC(1/2) | 0.949 | 0.599 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 278 | 0.3M Magnesium chloride hexahydrate, 0.3M Calcium chloride dihydrate, 1.0M Imidazole; MES monohydrate (acid) pH6.5, 25% MPD, 25% PEG 1000, 25% PEG 3350 |
