6U6J
RNA-monomer complex containing pyrophosphate linkage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2019-08-15 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 43.304, 43.304, 84.303 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.600 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6c8d |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.059 | 0.536 |
| Number of reflections | 12495 | 1234 |
| <I/σ(I)> | 44.9 | 4.2 |
| Completeness [%] | 98.9 | |
| Redundancy | 10.3 | 10.3 |
| CC(1/2) | 0.994 | 0.991 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 10mM MgCl2, 1.8 M Ammonium citrate tribasic pH 7.0 |
