6U6J

RNA-monomer complex containing pyrophosphate linkage

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	99
Detector technology	CCD
Collection date	2019-08-15
Detector	MAR CCD 130 mm
Wavelength(s)	1.0
Spacegroup name	P 3 2 1
Unit cell lengths	43.304, 43.304, 84.303
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.600
R-factor	0.23
Rwork	0.230
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6c8d
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.059	0.536
Number of reflections	12495	1234
<I/σ(I)>	44.9	4.2
Completeness [%]	98.9
Redundancy	10.3	10.3
CC(1/2)	0.994	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	10mM MgCl2, 1.8 M Ammonium citrate tribasic pH 7.0