6U55

Anti-Zaire ebolavirus Nucleoprotein Single Domain Antibody Zaire E (ZE) Complexed with Sudan ebolavirus Nucleoprotein C-terminal Domain 634-738

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	93.398, 28.298, 86.541
Unit cell angles	90.00, 114.45, 90.00

Refinement procedure

Resolution	46.260 - 1.930
R-factor	0.1995
Rwork	0.193
R-free	0.26310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6u51
RMSD bond length	0.008
RMSD bond angle	1.042
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.790	2.040
High resolution limit [Å]	1.930	1.930
Rmeas	0.088	1.690
Rpim	0.032	0.685
Number of reflections	11116	556
<I/σ(I)>	10.5	1
Completeness [%]	65.3	20.9
Redundancy	6.4	5.6
CC(1/2)	0.998	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.8 M ammonium sulfate, 0.1 M citric acid pH 3.5