6U53

Anti-Zaire ebolavirus Nucleoprotein Single Domain Antibody Zaire C (ZC)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 41 21 2
Unit cell lengths	78.414, 78.414, 37.811
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.240 - 1.490
R-factor	0.1938
Rwork	0.188
R-free	0.24910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6u51
RMSD bond length	0.009
RMSD bond angle	1.127
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.240	1.580
High resolution limit [Å]	1.490	1.490
Rmeas	0.043	1.970
Rpim	0.012	0.555
Number of reflections	13542	678
<I/σ(I)>	23.3	1.2
Completeness [%]	68.3	21.7
Redundancy	12.3	12.3
CC(1/2)	1.000	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	1.0 M sodium/potassium tartrate, 0.2 M lithium sulfate, 0.1M Tris pH 7.0