6U51

Anti-Sudan ebolavirus Nucleoprotein Single Domain Antibody Sudan B (SB) Complexed with Sudan ebolavirus Nucleoprotein C-terminal Domain 610-738

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 61 2 2
Unit cell lengths	117.881, 117.881, 144.959
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.940 - 2.520
R-factor	0.2124
Rwork	0.208
R-free	0.25040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6apo 6apq 4w2p
RMSD bond length	0.004
RMSD bond angle	0.832
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MR-Rosetta
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	145.000	2.660
High resolution limit [Å]	2.520	2.520
Rmeas	0.195	2.230
Rpim	0.057	0.660
Number of reflections	20678	2853
<I/σ(I)>	12.1	1
Completeness [%]	99.5	97
Redundancy	10.4	9.7
CC(1/2)	0.997	0.379

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	30% polyethylene glycol 3000, 0.1 M N-Cyclohexyl-2-aminoethanesulfonic acid (CHES) pH 9.5