6U51
Anti-Sudan ebolavirus Nucleoprotein Single Domain Antibody Sudan B (SB) Complexed with Sudan ebolavirus Nucleoprotein C-terminal Domain 610-738
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 117.881, 117.881, 144.959 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.940 - 2.520 |
| R-factor | 0.2124 |
| Rwork | 0.208 |
| R-free | 0.25040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6apo 6apq 4w2p |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.832 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MR-Rosetta |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 145.000 | 2.660 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmeas | 0.195 | 2.230 |
| Rpim | 0.057 | 0.660 |
| Number of reflections | 20678 | 2853 |
| <I/σ(I)> | 12.1 | 1 |
| Completeness [%] | 99.5 | 97 |
| Redundancy | 10.4 | 9.7 |
| CC(1/2) | 0.997 | 0.379 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 30% polyethylene glycol 3000, 0.1 M N-Cyclohexyl-2-aminoethanesulfonic acid (CHES) pH 9.5 |
