6U4K

Human talin2 residues 1-403

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-26
Detector	RAYONIX MX300HE
Wavelength(s)	1.0043
Spacegroup name	P 65
Unit cell lengths	58.885, 58.885, 203.799
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.996 - 2.555
R-factor	0.1977
Rwork	0.193
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	101.900	2.760
High resolution limit [Å]	2.555	2.555
Rpim	0.044	0.381
Number of reflections	12969	1876
<I/σ(I)>	15.7	2.7
Completeness [%]	100.0	100
Redundancy	10.1	5.7
CC(1/2)	0.998	0.734

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	8% PEG8000, Hepes pH 7.0