6U3Z

Structure of VD20_5A4 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	175.207, 108.733, 55.418
Unit cell angles	90.00, 105.78, 90.00

Refinement procedure

Resolution	84.304 - 1.990
R-factor	0.200763057575
Rwork	0.199
R-free	0.23632
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 5F9W 5dd5 2bx5
RMSD bond length	0.005
RMSD bond angle	0.968
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.310	2.061
High resolution limit [Å]	1.990	1.990
Rmerge	0.096	1.198
Rmeas	0.117
Rpim	0.065
Number of reflections	67170	6800
<I/σ(I)>	7.9	1.42
Completeness [%]	98.3	98.55
Redundancy	3	3.1
CC(1/2)		0.212

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	30% PEG3000, 0.2 M sodium chloride, 0.1 M Tris, pH 7, 0.01 M praseodymium(III) acetate hydrate, cryoprotectant: 26% glycerol