6U2Z
Crystal Structure of the metallo-beta-lactamase L1 from Stenotrophomonas maltophilia in the complex with the hydrolyzed moxalactam and two copper ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-21 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 104.234, 104.234, 98.793 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.340 - 2.380 |
| R-factor | 0.1877 |
| Rwork | 0.186 |
| R-free | 0.22920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 6U0Y |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.551 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.181 | 0.667 |
| Number of reflections | 13193 | 638 |
| <I/σ(I)> | 11.93 | 2.4 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.4 | 7.1 |
| CC(1/2) | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2 M Sodium malonate pH 7.0, 20% w/v Polyethylene glycol 3,350 |
