6U2T
Crystal structure of the T-state of maize C4-phosphoenolpyruvate carboxylase in complex with malate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.940, 140.931, 376.401 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.356 - 2.800 |
| R-factor | 0.1848 |
| Rwork | 0.183 |
| R-free | 0.22560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vyj |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 188.201 | 48.356 | 2.950 |
| High resolution limit [Å] | 2.800 | 8.850 | 2.800 |
| Rmerge | 0.035 | 0.404 | |
| Rmeas | 0.095 | 0.040 | 0.466 |
| Rpim | 0.046 | 0.021 | 0.226 |
| Total number of observations | 471354 | 14626 | 67401 |
| Number of reflections | 129366 | 4103 | 18754 |
| <I/σ(I)> | 10.5 | 25.6 | 3.2 |
| Completeness [%] | 95.3 | 90.3 | 95.5 |
| Redundancy | 3.6 | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 0.1 M TRI-SODIUM CITRATE//KOH, 0.2M AMMONIUM SULFATE, 14% PEG 4000 |
