6U0J
Crosslinked Crystal Structure of Malonyl-CoA Acyl Carrier Protein Transacylase, FabD, and Acyl Carrier Protein, AcpP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-03-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 73.150, 56.846, 99.874 |
Unit cell angles | 90.00, 97.84, 90.00 |
Refinement procedure
Resolution | 44.727 - 1.900 |
R-factor | 0.1865 |
Rwork | 0.185 |
R-free | 0.22130 |
Structure solution method | MOLECULAR REPLACEMENT |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.730 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.305 | 1.779 |
Rmeas | 0.329 | 1.927 |
Rpim | 0.122 | 0.732 |
Total number of observations | 231636 | |
Number of reflections | 32206 | 2091 |
<I/σ(I)> | 7.2 | |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 7.2 | 6.8 |
CC(1/2) | 0.988 | 0.468 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 281 | 1 % PEG 400, 0.1 M sodium cacodylate pH 6.5, and 2.5 M ammonium sulfate |