6U03

Crystal Structure of Fungal RNA Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	50.227, 55.667, 81.795
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.020 - 1.849
R-factor	0.1742
Rwork	0.170
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u32
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.880
High resolution limit [Å]	1.849	5.020	1.850
Rmerge	0.077	0.035	0.457
Rmeas	0.085	0.039	0.522
Rpim	0.036	0.017	0.248
Number of reflections	20105	1121	918
<I/σ(I)>	10.7
Completeness [%]	99.1	99.5	92.3
Redundancy	5.5	4.9	3.9
CC(1/2)		0.998	0.754

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.15M NaH2PO4, 20%PEG3350