6TZX

Crystal Structure of Fungal RNA Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97170
Spacegroup name	P 21 21 21
Unit cell lengths	56.650, 58.345, 72.321
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.410 - 1.529
R-factor	0.1759
Rwork	0.174
R-free	0.20790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u32
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.560
High resolution limit [Å]	1.529	4.150	1.530
Rmerge	0.078	0.040	0.349
Rmeas	0.088	0.045	0.464
Rpim	0.040	0.020	0.301
Number of reflections	36059	1993	1323
<I/σ(I)>	7.4
Completeness [%]	97.3	98	73.2
Redundancy	3.8	4.4	1.7
CC(1/2)		0.998	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.15M NaH2PO4, 20% PEG3350