6TZP
W96F Oxalate Decarboxylase (B. subtilis)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9787 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 155.073, 155.073, 123.079 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.865 - 1.720 |
| R-factor | 0.1615 |
| Rwork | 0.161 |
| R-free | 0.17870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vg3 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.247 | 1.760 |
| High resolution limit [Å] | 1.716 | 1.720 |
| Rmerge | 0.122 | 1.266 |
| Number of reflections | 60461 | 59982 |
| <I/σ(I)> | 19.32 | 2.31 |
| Completeness [%] | 99.9 | 99.22 |
| Redundancy | 4.3 | 13.8 |
| CC(1/2) | 0.490 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 20 mM Tris, 80 mM sodium chloride, 10 mM arginine, 10 mM glutamate, pH 7.0 |
