6TZO

Crystal Structure of Fungal RNA Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97170
Spacegroup name	P 21 21 21
Unit cell lengths	50.141, 56.427, 82.501
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.575 - 1.690
R-factor	0.1826
Rwork	0.180
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u32
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.690	4.610	1.700
Rmerge	0.059	0.033	0.524
Rmeas	0.064	0.036	0.592
Rpim	0.026	0.014	0.269
Number of reflections	25854	1455	1029
<I/σ(I)>	9.7
Completeness [%]	97.4	99	77
Redundancy	5.8	5.9	4
CC(1/2)		0.999	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.15M NaH2PO4, 20% PEG3350