6TZN

Structure of S. pombe telomerase accessory protein Pof8 C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 31 2 1
Unit cell lengths	57.360, 57.360, 70.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.675 - 1.350
R-factor	0.1662
Rwork	0.163
R-free	0.19400
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.713
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (1.3.1)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.675	49.675	1.390
High resolution limit [Å]	1.350	6.040	1.350
Rmerge	0.044	0.040	0.741
Rmeas	0.049	0.045	0.844
Total number of observations	136156
Number of reflections	29661	361	2148
<I/σ(I)>	16.09	34.49	1.95
Completeness [%]	99.1	91.6	98.6
Redundancy	4.59	4.427	4.236
CC(1/2)	0.999	0.998	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	14% PEG 3350, 0.1 M NaNO3, 0.1 M Tris