6TZM

Crystal Structure of Fungal RNA Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-02
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	50.562, 55.379, 81.419
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.791 - 1.714
R-factor	0.188
Rwork	0.186
R-free	0.20910
Structure solution method	MIR
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.740
High resolution limit [Å]	1.710	1.710
Rpim	0.035	0.227
Number of reflections	23506	660
<I/σ(I)>	32	2
Completeness [%]	93.0	52
Redundancy	4.6	2.7
CC(1/2)	0.994	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M MES pH 5, 30% PEG 6000- final pH 6