6TYN

Salmonella Typhi PltB Homopentamer with Neu-5NAc-9OAc-alpha-2-3-Gal-beta-1-4-GlcNAc Glycans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.87
Spacegroup name	P 21 21 21
Unit cell lengths	68.675, 97.747, 101.452
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rhr
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.390
High resolution limit [Å]	2.330	2.330
Rmerge	0.097	0.526
Number of reflections	27512	2036
<I/σ(I)>	37.4
Completeness [%]	99.6
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	26% PEG-1500, 0.1M Sodium Acetate pH 4.4