6TYD

Structure of human LDB1 in complex with SSBP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-03-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 65 2 2
Unit cell lengths	104.322, 104.322, 250.419
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.803 - 2.803
R-factor	0.2439
Rwork	0.240
R-free	0.28120
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	37297	1989
<I/σ(I)>	38.73
Completeness [%]	99.8	99.4
Redundancy	6.7	5.3
CC(1/2)	0.991	0.513

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	295	100 mM lithium sulfate monohydrate, 100 mM sodium citrate tribasic dihydrate pH 5.6, 1% v/v PEG400, 10mM DTT