6TY2
CT PART CRYSTAL STRUCTURE OF THE RYMV-ENCODED VIRAL RNA SILENCING SUPPRESSOR P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.253 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.081, 31.188, 41.980 |
| Unit cell angles | 90.00, 101.10, 90.00 |
Refinement procedure
| Resolution | 37.368 - 1.980 |
| R-factor | 0.1953 |
| Rwork | 0.194 |
| R-free | 0.22670 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.069 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.290 | 1.770 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.010 | 0.900 |
| Rmeas | 0.015 | |
| Rpim | 0.600 | |
| Number of reflections | 11192 | 1535 |
| <I/σ(I)> | 7 | 1.3 |
| Completeness [%] | 99.0 | 95 |
| Redundancy | 7 | 3 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M tri-Sodium citrate pH 5.6, 20 %(v/v) Isopropanol and 20 %(w/v) PEG 4000. |
