6TXS
The structure of the FERM domain and helical linker of human moesin bound to a CD44 peptide
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-25 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 41 |
| Unit cell lengths | 119.967, 119.967, 64.596 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 84.830 - 2.200 |
| Rwork | 0.232 |
| R-free | 0.27580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e5w |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.470 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.830 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.166 | 3.189 |
| Rmeas | 0.173 | 3.492 |
| Rpim | 0.048 | 1.372 |
| Number of reflections | 23382 | 1977 |
| <I/σ(I)> | 11.1 | 0.6 |
| Completeness [%] | 99.7 | 96.4 |
| Redundancy | 23.7 | 11.7 |
| CC(1/2) | 0.999 | 0.460 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.2M potassium thiocyanate, 0.1M bis-tris pH 7.0, 10% ethylene glycol, 20% PEG3350 |
