6TXS

The structure of the FERM domain and helical linker of human moesin bound to a CD44 peptide

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	I 41
Unit cell lengths	119.967, 119.967, 64.596
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	84.830 - 2.200
Rwork	0.232
R-free	0.27580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e5w
RMSD bond length	0.007
RMSD bond angle	1.470
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.830	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.166	3.189
Rmeas	0.173	3.492
Rpim	0.048	1.372
Number of reflections	23382	1977
<I/σ(I)>	11.1	0.6
Completeness [%]	99.7	96.4
Redundancy	23.7	11.7
CC(1/2)	0.999	0.460

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.2M potassium thiocyanate, 0.1M bis-tris pH 7.0, 10% ethylene glycol, 20% PEG3350