6TXM

Crystal structure of thermotoga maritima E65R Ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	H 3 2
Unit cell lengths	176.262, 176.262, 353.783
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.979 - 2.198
R-factor	0.2047
Rwork	0.204
R-free	0.24330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vlg
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.15)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.979	47.979	2.330
High resolution limit [Å]	2.198	6.540	2.200
Rmerge	0.216	0.076	2.151
Rmeas	0.228	0.080	2.269
Number of reflections	107042	4273	17052
<I/σ(I)>	7.52	26.31	0.83
Completeness [%]	99.8	99.5	99.3
Redundancy	10.24	9.864	9.858
CC(1/2)	0.996	0.997	0.403

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.4 M (NH4)2 SO4, 0.1M MES. pH 6.0 10mg/ml protein in 20mM HEPES, 150mM NaCl. Mixed in 1:1 ratio.