6TXL

Crystal structure of thermotoga maritima E65Q Ferritin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-13
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	0.9184
Spacegroup name	H 3 2
Unit cell lengths	176.219, 176.219, 355.045
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.884 - 2.099
R-factor	0.193
Rwork	0.193
R-free	0.22180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vlg
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.15.1_3469)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.884	26.884	2.220
High resolution limit [Å]	2.099	6.240	2.100
Rmerge	0.152	0.043	2.570
Rmeas	0.160	0.045	2.708
Number of reflections	123375	4909	19455
<I/σ(I)>	10.27	41.25	0.7
Completeness [%]	99.6	99.5	98
Redundancy	10.043	9.262	9.974
CC(1/2)	0.998	0.999	0.331

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.4 M (NH4)2 SO4, 0.1M MES. pH 6.0. 10mg/ml protein in 20mM HEPES, 150mM NaCl. Mixed 1:1 ratio