Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9159 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.542, 105.587, 112.405 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.266 - 1.350 |
| Rwork | 0.143 |
| R-free | 0.17760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vua |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.743 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 112.410 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 104434 | 5129 |
| <I/σ(I)> | 10.7 | 0.9 |
| Completeness [%] | 100.0 | |
| Redundancy | 10.5 | |
| CC(1/2) | 0.999 | 0.463 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.2 M Sodium acetate trihydrate, 0.1 M Bis-Tris propane-HCl pH 7.5, 22% w/v PEG 3350 |
