6TV6
Octameric McsB from Bacillus subtilis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 137.790, 147.640, 81.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.900 - 2.500 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fh1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.083 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.900 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmeas | 0.146 | 0.153 |
| Number of reflections | 58145 | 4251 |
| <I/σ(I)> | 13.6 | 1.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 6.7 | 6.9 |
| CC(1/2) | 0.998 | 0.561 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 200 mM Mg-acetate |
