6TV6

Octameric McsB from Bacillus subtilis.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	137.790, 147.640, 81.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.900 - 2.500
R-factor	0.195
Rwork	0.195
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fh1
RMSD bond length	0.009
RMSD bond angle	1.083
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.900	2.560
High resolution limit [Å]	2.500	2.500
Rmeas	0.146	0.153
Number of reflections	58145	4251
<I/σ(I)>	13.6	1.2
Completeness [%]	99.8	99.8
Redundancy	6.7	6.9
CC(1/2)	0.998	0.561

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277.15	200 mM Mg-acetate