6TV2
Heme d1 biosynthesis associated Protein NirF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-28 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 101.129, 147.830, 108.716 |
Unit cell angles | 90.00, 98.21, 90.00 |
Refinement procedure
Resolution | 59.460 - 1.561 |
R-factor | 0.1648 |
Rwork | 0.163 |
R-free | 0.20240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rte |
Data reduction software | DIALS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASES |
Refinement software | PHENIX (dev-3742) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 107.600 | 1.730 |
High resolution limit [Å] | 1.561 | 1.561 |
Rpim | 0.067 | 0.394 |
Number of reflections | 289596 | 14420 |
<I/σ(I)> | 6.4 | 1.7 |
Completeness [%] | 94.7 | 69.3 |
Redundancy | 3.7 | 3.8 |
CC(1/2) | 0.995 | 0.655 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10% (w/v) PEG 4000 20% (v/v)glycerol 0.03 NaNO3 0.03 Na2HPO4 0.03 (NH4)2SO4 0.1M MOPS/HEPES pH7.5 |