6TUN

Helicase domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0064
Spacegroup name	P 21 21 21
Unit cell lengths	74.940, 78.350, 92.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.710 - 2.070
R-factor	0.1933
Rwork	0.192
R-free	0.22730
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.888
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.710	2.130
High resolution limit [Å]	2.070	2.070
Rpim	0.038	0.439
Number of reflections	33675	2591
<I/σ(I)>	11.6	2
Completeness [%]	99.6
Redundancy	5.4
CC(1/2)	0.999	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	100 mM Tris pH 8.5 25% PEG8000