6TUL
Structure of the arginase-2-inhibitory human antigen-binding fragment Fab C0021177
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.537, 69.244, 106.517 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.505 - 2.250 |
| Rwork | 0.248 |
| R-free | 0.31760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6srv |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.218 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.830 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.180 | 4.040 |
| Number of reflections | 19456 | 1755 |
| <I/σ(I)> | 8.4 | 0.7 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 12.8 | 13.3 |
| CC(1/2) | 1.000 | 0.360 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 100 mM MMT (D/L-malic acid, MES, tris) 25% PEG1500 |
