6TTY
Structure of ClpP from Staphylococcus aureus (apo, closed state)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 95.190, 99.250, 107.470 |
| Unit cell angles | 105.22, 101.27, 117.70 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.1751 |
| Rwork | 0.173 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v5e |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.051 | 0.540 |
| Number of reflections | 215068 | 30904 |
| <I/σ(I)> | 9 | 2 |
| Completeness [%] | 90.3 | |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM MES, 0.2 M MgAc, 15% MPD |
