Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.572, 64.472, 67.590 |
| Unit cell angles | 96.35, 101.95, 97.27 |
Refinement procedure
| Resolution | 32.730 - 1.900 |
| Rwork | 0.169 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fat |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.846 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.732 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.073 | 0.529 |
| Rpim | 0.049 | 0.372 |
| Number of reflections | 73400 | 4491 |
| <I/σ(I)> | 11 | 2.8 |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 6.2 | 5.7 |
| CC(1/2) | 0.997 | 0.902 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 15% Peg smear low 100mM Tris pH 7.5 PEG to 30% for cryoprotection |
