6TSY

Cytochrome c6 from Thermosynechococcus elongatus in a monoclinic space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.00000
Spacegroup name	C 1 2 1
Unit cell lengths	106.019, 109.905, 55.402
Unit cell angles	90.00, 100.89, 90.00

Refinement procedure

Resolution	41.760 - 2.250
R-factor	0.191
Rwork	0.188
R-free	0.25120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eic
RMSD bond length	0.014
RMSD bond angle	1.269
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.760	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.150	1.570
Rmeas	0.190	1.880
Rpim	0.100	1.010
Number of reflections	28317	2409
<I/σ(I)>	9.93	1.8
Completeness [%]	95.5	80.72
Redundancy	3.2	3.4
CC(1/2)	0.920	0.466

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	2M ammonium sulfate, 40mM KNO3