Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.28242 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 121.071, 121.071, 99.624 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.400 - 1.850 |
| Rwork | 0.207 |
| R-free | 0.23470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ef2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.811 |
| Data reduction software | XDS (Mar 15, 2019 BUILT=20190315) |
| Data scaling software | XDS (Mar 15, 2019 BUILT=20190315) |
| Phasing software | PHASER (7.0.078) |
| Refinement software | REFMAC (7.0.078) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.066 | 1.437 |
| Rmeas | 0.073 | 1.767 |
| Rpim | 0.031 | 0.986 |
| Number of reflections | 64833 | 1507 |
| <I/σ(I)> | 18.2 | |
| Completeness [%] | 88.2 | |
| Redundancy | 4.4 | |
| CC(1/2) | 0.999 | 0.489 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.1 M cacodylate/HCl pH 6.5, 16% PEG 8000, 0.5 M sodium acetate, 50 mM ZnCl2, 10 mM CaCl2, 12.5% DMSO, 5 mM DTT |
