6TRR

Structural insight into tanapoxvirus mediated inhibition of apoptosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 43 21 2
Unit cell lengths	55.376, 55.376, 126.945
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.622 - 2.120
R-factor	0.240417513652
Rwork	0.240
R-free	0.25419
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uf1
RMSD bond length	0.003
RMSD bond angle	0.436
Data reduction software	XDS
Data scaling software	Aimless (7.0.077)
Phasing software	PHASER (1.11.1_2575)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.310	2.180
High resolution limit [Å]	2.120	2.120
Rmerge	0.097
Number of reflections	11903	946
<I/σ(I)>	11.2
Completeness [%]	100.0
Redundancy	8.6
CC(1/2)	0.999	0.291

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	1.0 M Lithium Chloride, 0.1 M Citrate pH 4.0 20% PEG 6000