6TQQ
Structural insight into tanapoxvirus mediated inhibition of apoptosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 59.654, 59.654, 90.908 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.662 - 3.002 |
| R-factor | 0.243997220214 |
| Rwork | 0.242 |
| R-free | 0.27638 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Previously solved structure of TNP_Bax |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.554 |
| Data reduction software | XDS |
| Data scaling software | Aimless (7.0.077) |
| Phasing software | PHASER (1.11.1_2575) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.662 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.168 | 0.168 |
| Number of reflections | 4030 | 4030 |
| <I/σ(I)> | 4.3 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.7 | |
| CC(1/2) | 0.996 | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M MIB pH 8.0, 25% PEG 1500 |
