6TQ9
Crystal structure of the Orexin-1 receptor in complex with SB-408124
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.365, 147.067, 72.536 |
Unit cell angles | 90.00, 111.09, 90.00 |
Refinement procedure
Resolution | 44.713 - 2.655 |
R-factor | 0.241 |
Rwork | 0.240 |
R-free | 0.26930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6to7 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.713 | 2.795 |
High resolution limit [Å] | 2.655 | 2.655 |
Number of reflections | 19851 | 297 |
<I/σ(I)> | 8.7 | |
Completeness [%] | 58.3 | |
Redundancy | 9.6 | |
CC(1/2) | 0.998 | 0.562 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 284 | 0.1M TRISODIUM CITRATE 50mM SODIUM CHLORIDE 50mM LITHIUM SULPHATE 15-34% PEG400 |